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The following diagram indicates the cutting sites of three different restriction enzymes on a particular bacterial plasmid - VCE - SSCE Biology - Question 16 - 2005 - Paper 1
Question 16
The following diagram indicates the cutting sites of three different restriction enzymes on a particular bacterial plasmid. If the plasmid was incubated with the re... show full transcript
Worked Solution & Example Answer:The following diagram indicates the cutting sites of three different restriction enzymes on a particular bacterial plasmid - VCE - SSCE Biology - Question 16 - 2005 - Paper 1
Step 1
If the plasmid was incubated with the restriction enzyme Eco R1, the number of pieces of DNA obtained would be
Answer
To determine the number of DNA pieces produced when the plasmid is cut by the Eco R1 enzyme, we should analyze the cutting sites indicated in the diagram.
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Identifying Cutting Sites: From the diagram, we locate the Eco R1 restriction sites. We see two Eco R1 cutting sites on the plasmid.
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Cutting the Plasmid: When both Eco R1 sites cut the plasmid, they will effectively split the circle into three separate pieces of DNA. Each restriction site creates a break in the circular DNA.
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Conclusion: Thus, after the action of Eco R1, the number of distinct pieces of DNA obtained from the plasmid is four.
Therefore, the correct answer is C.