The diagram below represents a DNA molecule and the position of the recognition sites for the restriction enzymes BamHI, EcoRI, HaeIII and Sall - VCE - SSCE Biology - Question 38 - 2017 - Paper 1
Question 38
The diagram below represents a DNA molecule and the position of the recognition sites for the restriction enzymes BamHI, EcoRI, HaeIII and Sall.
Also shown is a dia... show full transcript
Worked Solution & Example Answer:The diagram below represents a DNA molecule and the position of the recognition sites for the restriction enzymes BamHI, EcoRI, HaeIII and Sall - VCE - SSCE Biology - Question 38 - 2017 - Paper 1
Step 1
R: Which enzyme was used?
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Answer
Lane R shows a single band, indicating that a single enzyme was likely used to digest the DNA. The band pattern suggests that EcoRI, which gives a more defined cut, was used here.
Step 2
S: Which enzyme was used?
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Lane S shows two bands, which suggests that HaeIII was the enzyme used. HaeIII is known for producing fragments that are smaller in size, which aligns with the observed pattern.
Step 3
T: Which enzyme was used?
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Lane T shows a larger fragment, which is likely indicative of the BamHI enzyme being used. BamHI cuts at a specific sequence that typically leaves larger fragments.
Step 4
U: Which enzyme was used?
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Lane U also presents only one fragment, suggesting that Sall was the enzyme used in this case, producing a distinct and identifiable band pattern.
