What is a probe?
DNA microarray technology, also known as DNA chip technology, allows screening to detect mutations - VCE - SSCE Biology - Question 7 - 2009 - Paper 1
Question 7
What is a probe?
DNA microarray technology, also known as DNA chip technology, allows screening to detect mutations. A DNA chip is made of glass and can contain tho... show full transcript
Worked Solution & Example Answer:What is a probe?
DNA microarray technology, also known as DNA chip technology, allows screening to detect mutations - VCE - SSCE Biology - Question 7 - 2009 - Paper 1
Step 1
What is a probe?
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Answer
A probe is a single-stranded segment of DNA that is (radioactively) labelled. It functions to indicate hybridization with a normal allele or to highlight specific mutations in the target DNA.
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In this step, we treat a normal allele of p53 to break it down into its nucleotide sequences, facilitating the further testing of mutations.
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Each nucleotide segment of the p53 gene is tested individually. In the case of nucleotide five [G], we manufacture sequences to examine all possible mutations, comparing each against the normal sequence to determine any alterations.
