The diagram below represents a DNA molecule and the position of the recognition sites for the restriction enzymes BamHI, EcoRI, HaeIII and SalI - VCE - SSCE Biology - Question 38 - 2017 - Paper 1
Question 38
The diagram below represents a DNA molecule and the position of the recognition sites for the restriction enzymes BamHI, EcoRI, HaeIII and SalI.
DNA molecule
BamHI ... show full transcript
Worked Solution & Example Answer:The diagram below represents a DNA molecule and the position of the recognition sites for the restriction enzymes BamHI, EcoRI, HaeIII and SalI - VCE - SSCE Biology - Question 38 - 2017 - Paper 1
Step 1
R
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Answer
The lane R shows a band pattern that matches with the action of EcoRI. EcoRI cuts at specific recognition sites within the DNA sequence, facilitating a unique banding pattern seen in lane R.
Step 2
S
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The bands in lane S correspond to the action of HaeIII. HaeIII is known for producing fragments of a specific size, allowing us to identify it as the enzyme responsible for the segments in this lane.
Step 3
T
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The lane T matches the cutting pattern of BamHI. BamHI generates a distinct banding pattern that can be recognized as the output of this specific restriction enzyme.
Step 4
U
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Finally, the lane U represents the digestion by SalI. The size of the bands produced here indicates that SalI was the enzyme used, as it cuts at a specific site in the DNA.
