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To make the mRNA molecule containing the target sequence, a cell must: - synthesize DNA polymerase - VCE - SSCE Biology - Question 2 - 2021 - Paper 1

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To make the mRNA molecule containing the target sequence, a cell must: - synthesize DNA polymerase. - attach a poly-A tail to the 3' end of the gene. - create a nucl... show full transcript

Worked Solution & Example Answer:To make the mRNA molecule containing the target sequence, a cell must: - synthesize DNA polymerase - VCE - SSCE Biology - Question 2 - 2021 - Paper 1

Step 1

synthesize DNA polymerase

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Answer

To synthesize the mRNA molecule, the cell must first produce DNA polymerase, which is essential for transcribing DNA into RNA. This enzyme catalyzes the polymerization of nucleotides to form the mRNA strand.

Step 2

attach a poly-A tail to the 3' end of the gene

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Answer

Once the mRNA strand is synthesized, a poly-A tail is added to the 3' end. This modification enhances mRNA stability and facilitates its export from the nucleus.

Step 3

create a nucleotide chain with a ribose–phosphate backbone

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Answer

The creation of a nucleotide chain involves linking ribose sugars and phosphate groups to form the backbone of the mRNA. Each nucleotide is joined by phosphodiester bonds between the 5' phosphate group of one nucleotide and the 3' hydroxyl group of another.

Step 4

have a ready supply of the N-containing bases adenine, thymine, guanine and cytosine

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Answer

The cell must maintain a supply of nitrogenous bases: adenine (A), uracil (U), guanine (G), and cytosine (C) for RNA synthesis, where thymine (T) is not used in RNA. This supply ensures that the mRNA can be properly synthesized according to the DNA template.

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