The following diagram indicates the cutting sites of three different restriction enzymes on a particular bacterial plasmid - VCE - SSCE Biology - Question 16 - 2005 - Paper 1
Question 16
The following diagram indicates the cutting sites of three different restriction enzymes on a particular bacterial plasmid.
If the plasmid was incubated with the re... show full transcript
Worked Solution & Example Answer:The following diagram indicates the cutting sites of three different restriction enzymes on a particular bacterial plasmid - VCE - SSCE Biology - Question 16 - 2005 - Paper 1
Step 1
Determine the cut sites for Eco R1
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Answer
Identify the positions of Eco R1 in the diagram. There are two Eco R1 cut sites indicated on the plasmid.
Step 2
Analyze the effect of cuts on the plasmid
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Answer
Since Eco R1 cuts the plasmid at two locations, it will divide the plasmid into segments. The number of pieces of DNA obtained from a circular plasmid after two cuts will be calculated as follows:
A circular plasmid without cuts is one piece.
Each cut creates an additional piece:
1 (initial piece) + 2 (cuts) = 3 pieces.
Step 3
Conclusion
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Answer
Therefore, after incubation with Eco R1, the plasmid will yield three pieces of DNA. The correct answer is B. three.
