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Cells can be ruptured and their contents collected after a series of centrifugation at different speeds - VCE - SSCE Biology - Question 5 - 2009 - Paper 1
Question 5
Cells can be ruptured and their contents collected after a series of centrifugation at different speeds. The largest components sediment and form a pellet at a relat... show full transcript
Worked Solution & Example Answer:Cells can be ruptured and their contents collected after a series of centrifugation at different speeds - VCE - SSCE Biology - Question 5 - 2009 - Paper 1
Step 1
The sequence of pellets obtained in tubes 1, 2, 3 and 4 would be:
Answer
To determine the sequence of pellets, we need to understand the order of sedimentation based on the centrifugation speeds outlined. The first tube sedimented at 500 relative speed will have the largest components, which are typically the nuclei, followed by the endoplasmic reticulum, and smaller components such as mitochondria and ribosomes in the subsequent tubes. Therefore, the correct sequence of pellets is:
C. nuclei, mitochondria, endoplasmic reticulum, ribosomes.