Victoria Police forensic scientists conduct DNA profiling using samples taken from crime scenes - VCE - SSCE Biology - Question 2 - 2007 - Paper 1
Question 2
Victoria Police forensic scientists conduct DNA profiling using samples taken from crime scenes. Traces of DNA of less than 1 nanogram can be amplified and then prof... show full transcript
Worked Solution & Example Answer:Victoria Police forensic scientists conduct DNA profiling using samples taken from crime scenes - VCE - SSCE Biology - Question 2 - 2007 - Paper 1
Step 1
a. Name the process which is used to amplify the DNA.
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Answer
The process used to amplify DNA is called Polymerase Chain Reaction (PCR).
Step 2
b. What must be done between stages 1 and 2 to separate the strands of the DNA molecule?
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Answer
To separate the strands of the DNA molecule, the temperature must be increased to 90°C or greater in order to dissociate the strands and break the hydrogen bonds.
Step 3
c. Complete and label the diagram at stage 2.
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In stage 2, the diagram should include the following:
Primers attached at the 3' end on opposite strands.
The temperature should be reduced to approximately 50°C.
DNA/Taq polymerase is added to propagate the process away from the primer.
