The following figures show two different methods used to sequence DNA - VCE - SSCE Biology - Question 7 - 2011 - Paper 1
Question 7
The following figures show two different methods used to sequence DNA.
Method 1
result – different colours
C T A C T A G G
migration of DNA fragments
detector
l... show full transcript
Worked Solution & Example Answer:The following figures show two different methods used to sequence DNA - VCE - SSCE Biology - Question 7 - 2011 - Paper 1
Step 1
A. method 1 requires 4 slots for the loading of the DNA.
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Answer
This statement is incorrect. Method 1 involves migration through a buffer, which does not explicitly indicate a requirement for 4 slots.
Step 2
B. method 2 uses a laser beam to separate the individual nucleotides.
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Answer
This statement is also incorrect. Method 2 involves a gel setup and not the use of a laser beam for separation.
Step 3
C. method 1 uses fluorescent dyes to distinguish between the different nucleotides.
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Answer
This statement is correct. Method 1 employs fluorescent dyes to differentiate the nucleotides as indicated by the different colors observed in the results.
Step 4
D. method 2 requires the DNA to be loaded at the positive end on the sequencing gel.
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Answer
This statement is incorrect. The question indicates a gel setup, but it does not specify that loading is required at the positive end.
