Which one of the following would be a requirement of either Step 1 or Step 2?
- Step 2 would require the use of a thermocycling machine to maintain the temperature at 37 °C - VCE - SSCE Biology - Question 30 - 2021 - Paper 1
Question 30
Which one of the following would be a requirement of either Step 1 or Step 2?
- Step 2 would require the use of a thermocycling machine to maintain the temperature ... show full transcript
Worked Solution & Example Answer:Which one of the following would be a requirement of either Step 1 or Step 2?
- Step 2 would require the use of a thermocycling machine to maintain the temperature at 37 °C - VCE - SSCE Biology - Question 30 - 2021 - Paper 1
Step 1
Step 2 would require the use of a thermocycling machine to maintain the temperature at 37 °C.
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Answer
This statement is true, as Step 2 typically involves processes such as PCR (Polymerase Chain Reaction) that require precise temperature control to allow for denaturation, annealing, and extension phases.
Step 2
Step 1 would require the quill samples to have been stored at a very high temperature.
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Answer
This statement does not align with standard procedures, as quill samples would usually be stored at low temperatures to preserve DNA integrity.
Step 3
Step 1 would require the extraction of DNA from the nuclei of quill epithelial cells.
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Answer
This statement is accurate as Step 1 involves the isolation of DNA, which typically requires accessing the nuclei of cells.
Step 4
Step 2 would usually require a reaction mixture containing two primers.
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Answer
This statement is correct, as PCR processes in Step 2 indeed require a set of primers to target specific DNA sequences for amplification.
