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a. What term is used to describe the ends of the fragments produced by MstII? Molecular studies have shown that the sickle cell allele differs only by one base pair from the normal allele - VCE - SSCE Biology - Question 7 - 2004 - Paper 1
Question 7
a. What term is used to describe the ends of the fragments produced by MstII? Molecular studies have shown that the sickle cell allele differs only by one base pair... show full transcript
Worked Solution & Example Answer:a. What term is used to describe the ends of the fragments produced by MstII? Molecular studies have shown that the sickle cell allele differs only by one base pair from the normal allele - VCE - SSCE Biology - Question 7 - 2004 - Paper 1
Step 1
What term is used to describe the ends of the fragments produced by MstII?
Answer
The term used to describe the ends of the fragments produced by MstII is 'sticky ends'. Sticky ends refer to the short, single-strand overhangs generated after the MstII enzyme cuts the DNA at its recognition site.
Step 2
How is the action of the MstII enzyme affected by the HbS mutation?
Answer
The action of the MstII enzyme is affected by the HbS mutation because the enzyme does not cut the DNA at the modified site. This happens because the HbS mutation alters the base pair sequence recognized by MstII, preventing the enzyme from binding and recognizing the cut site.