To determine the uptake of resistance genes by the wheat plants, scientists investigate gene fragments using electrophoresis to
A - VCE - SSCE Biology - Question 10 - 2023 - Paper 1
Question 10
To determine the uptake of resistance genes by the wheat plants, scientists investigate gene fragments using electrophoresis to
A. cut the DNA.
B. amplify the DNA.
C... show full transcript
Worked Solution & Example Answer:To determine the uptake of resistance genes by the wheat plants, scientists investigate gene fragments using electrophoresis to
A - VCE - SSCE Biology - Question 10 - 2023 - Paper 1
Step 1
A. cut the DNA.
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Answer
This step involves using restriction enzymes to cut DNA at specific sites, allowing the fragments to be analyzed. However, this is not the primary purpose of electrophoresis.
Step 2
B. amplify the DNA.
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Amplification is typically achieved through PCR (Polymerase Chain Reaction) before electrophoresis. Thus, it's not directly associated with the role of electrophoresis itself.
Step 3
C. separate the DNA.
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Electrophoresis is specifically designed to separate DNA fragments based on size. The gel matrix allows smaller fragments to migrate further than larger ones, which is the main purpose in analyzing gene fragments.
Step 4
D. denature the DNA.
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Denaturation involves the separation of DNA strands, usually before PCR. While it can happen before gel electrophoresis, it is not the primary role of electrophoresis.
