Aspartame is an ingredient in some soft drinks - VCE - SSCE Chemistry - Question 9 - 2021 - Paper 1
Question 9
Aspartame is an ingredient in some soft drinks. Aspartame is unstable in some conditions and reacts to form four main products. One of the products of aspartame brea... show full transcript
Worked Solution & Example Answer:Aspartame is an ingredient in some soft drinks - VCE - SSCE Chemistry - Question 9 - 2021 - Paper 1
Step 1
9bi. rate of production of DKP
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Answer
The rate of production of DKP from aspartame can be analyzed by comparing the concentration of DKP over time in the samples analyzed from lemonade. This involves measuring the peak area associated with DKP in the chromatograms obtained through HPLC.
Step 2
9bi. change in concentration of DKP (over time)
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Answer
To determine the change in concentration of DKP over time, a calibration curve must first be generated using known concentrations of DKP. The peak areas from the collected samples can then be plotted against the concentrations from the calibration curve. This will enable the calculation of the varying concentrations of DKP over the three-day period.
Step 3
9bi. change in amount of DKP (over time)
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Answer
The amount of DKP can be calculated by multiplying the concentration of DKP found in each sample by the total volume of the lemonade analyzed. This allows for tracking how much DKP is produced over the designated time intervals.
Step 4
9ci. increasing the height of the column
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Answer
To improve separation in HPLC, increasing the height of the column can enhance the resolution of the peaks, allowing for more accurate quantification of aspartame and DKP.
Step 5
9ci. altering either the mobile or stationary phases
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Adjusting the composition of the mobile phase or the properties of the stationary phase can affect the interaction between the analytes and the column, thereby influencing the separation process.
Step 6
9ci. altering the temperature of the column
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Answer
Changing the temperature of the column can affect the viscosity of the mobile phase and the interaction kinetics, impacting the overall separation and resolution of the chromatographic run.
