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Gel electrophoresis is a technique that can be used to separate DNA fragments in forensic chemistry - VCE - SSCE Chemistry - Question 11 - 2008 - Paper 1
Question 11
Gel electrophoresis is a technique that can be used to separate DNA fragments in forensic chemistry. The gel resulting from such a separation experiment carried out ... show full transcript
Worked Solution & Example Answer:Gel electrophoresis is a technique that can be used to separate DNA fragments in forensic chemistry - VCE - SSCE Chemistry - Question 11 - 2008 - Paper 1
Step 1
Which of the following statements about the experiment is not correct?
Answer
To determine the statement that is not correct, we need to analyze each option:
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A. Fragment X has a higher molecular mass than fragment Y. This may be true if fragment X appears larger on the gel, indicating it is heavier. However, this is not an incorrect statement by itself.
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B. Fragment Y moves through the gel at a faster rate than fragment X. This is typically true as smaller fragments move faster through the gel than larger ones.
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C. The negative terminal of the power supply is connected to end A of the gel. This statement is correct because DNA is negatively charged and moves towards the positive terminal, typically located at the opposite end.
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D. Under the conditions of this experiment, the DNA fragments are positively charged. This statement is false. DNA fragments are negatively charged due to the phosphate backbone and migrate towards the positive electrode.
Based on the analysis, the incorrect statement is D.