Chromatogram 1 was obtained by analysis of a sample of a mixture of two sugars, A and B, using high-performance liquid chromatography (HPLC) - VCE - SSCE Chemistry - Question 5 - 2007 - Paper 1
Question 5
Chromatogram 1 was obtained by analysis of a sample of a mixture of two sugars, A and B, using high-performance liquid chromatography (HPLC). Chromatogram 2 was obta... show full transcript
Worked Solution & Example Answer:Chromatogram 1 was obtained by analysis of a sample of a mixture of two sugars, A and B, using high-performance liquid chromatography (HPLC) - VCE - SSCE Chemistry - Question 5 - 2007 - Paper 1
Step 1
I decreasing the pressure of the mobile phase
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Answer
Decreasing the pressure of the mobile phase typically increases the retention time for compounds in HPLC. This suggests a shift towards slower migration for sugars A and B, which would be unlikely to produce chromatogram 2.
Step 2
II decreasing the temperature
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Answer
Decreasing the temperature can affect the viscosity of the mobile phase and may slow down the separation process. However, in many cases, this results in sharper peaks, potentially aligning with chromatogram 2's quicker separation.
Step 3
III using a less tightly packed column
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Answer
Using a less tightly packed column increases the flow rate of the mobile phase, allowing for a faster elution of components. This could explain the shorter retention times seen in chromatogram 2, making it a viable candidate.
