An aqueous mixture of two substances (Y and Z) is subjected to analysis by both paper chromatography and high performance liquid chromatography (HPLC). In both forms of chromatography, component Z of the mixture was bonded more strongly to the stationary phase than component Y. In terms of $R_f$ and $R_t$, where $R_t$ is the retention time in HPLC, component Z has the A. higher $R_f$, higher $R_t$ B. higher $R_f$, lower $R_t$ C. lower $R_f$, higher $R_t$ D. lower $R_f$, lower $R_t$

SSCE VCE Chemistry Instrumental Analysis of Organic Compounds: An aqueous mixture of two substa

An aqueous mixture of two substances (Y and Z) is subjected to analysis by both paper chromatography and high performance liquid chromatography (HPLC) - VCE - SSCE Chemistry - Question 3 - 2004 - Paper 1

Question 3

An aqueous mixture of two substances (Y and Z) is subjected to analysis by both paper chromatography and high performance liquid chromatography (HPLC). In both forms... show full transcript

Worked Solution & Example Answer:An aqueous mixture of two substances (Y and Z) is subjected to analysis by both paper chromatography and high performance liquid chromatography (HPLC) - VCE - SSCE Chemistry - Question 3 - 2004 - Paper 1

Step 1

In terms of $R_f$ and $R_t$, where $R_t$ is the retention time in HPLC, component Z has the:

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Answer

In chromatography, the retention factor ( $R_f$ ) indicates how strongly a component is bonded to the stationary phase. A lower $R_f$ value suggests that the component is more strongly adsorbed to the stationary phase compared to others. Since component Z is bonded more strongly than component Y, we can infer that component Z has a lower $R_f$ value than component Y.

In high performance liquid chromatography (HPLC), the retention time ( $R_t$ ) is inversely related to how strongly a substance interacts with the stationary phase. Thus, if component Z is more strongly bonded to the stationary phase, it will experience a longer retention time, resulting in a higher $R_t$ .

Therefore, the correct answer is C: lower $R_f$ , higher $R_t$ .

An aqueous mixture of two substances (Y and Z) is subjected to analysis by both paper chromatography and high performance liquid chromatography (HPLC) - VCE - SSCE Chemistry - Question 3 - 2004 - Paper 1

Worked Solution & Example Answer:An aqueous mixture of two substances (Y and Z) is subjected to analysis by both paper chromatography and high performance liquid chromatography (HPLC) - VCE - SSCE Chemistry - Question 3 - 2004 - Paper 1

In terms of $R_f$ and $R_t$, where $R_t$ is the retention time in HPLC, component Z has the:

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