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1. What is meant by a genome? 2 - AQA - A-Level Biology - Question 8 - 2018 - Paper 1
Question 8
1. What is meant by a genome? 2. Explain why the antibody binds to the transcription factor. 3. Use Figure 8 to explain what 'precipitated DNA' consists of. Soybe... show full transcript
Worked Solution & Example Answer:1. What is meant by a genome? 2 - AQA - A-Level Biology - Question 8 - 2018 - Paper 1
Step 1
Step 2
Explain why the antibody binds to the transcription factor.
Answer
The antibody binds to the transcription factor because they have a specific tertiary structure that allows for complementary binding. This specific shape enables the antibody to recognize and attach to the transcription factor, thus allowing for biological interactions.
Step 3
Step 4
Suggest how single-stranded cDNA could prevent transcription of the P34 gene.
Answer
Single-stranded cDNA could prevent transcription of the P34 gene by binding to the corresponding mRNA that is normally transcribed from the P34 gene. This binding would then inhibit the interaction between the mRNA and the ribosome, thus preventing translation into the P34 protein.
Step 5
Describe the roles of two named types of enzymes used to insert DNA fragments into plasmids.
Answer
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Restriction enzymes (endonucleases) are used to cut the plasmid at specific sites, allowing for the insertion of the DNA fragments.
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Ligase is then used to join the DNA fragment with the plasmid, forming a stable recombinant DNA molecule.
Step 6
Suggest two features of the structure of different proteins that enable them to be separated by gel electrophoresis.
Answer
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Mass or size of amino acids or polypeptides affects how fast the proteins move through the gel.
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Charge of the proteins, which influences their movement in an electric field; differences in charge lead to varying migration rates.