Catalase is an enzyme - AQA - GCSE Biology - Question 2 - 2018 - Paper 1
Question 2
Catalase is an enzyme.
Catalase controls the following reaction:
hydrogen peroxide → water + oxygen
A student did an investigation on catalase activity.
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Worked Solution & Example Answer:Catalase is an enzyme - AQA - GCSE Biology - Question 2 - 2018 - Paper 1
Step 1
Circle the anomaly in Table 1.
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Answer
The anomaly in Table 1 is 0.0 at 20 °C. This value is significantly lower compared to the other results at adjacent temperatures.
Step 2
What conclusion can be made as the temperature increases?
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Answer
The conclusion that can be made is that the rate of reaction increases up to 30 °C.
Step 3
At which temperature was catalase denatured?
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Answer
Catalase was denatured at 60 °C.
Step 4
How could the investigation be improved to find a more precise value for the optimum temperature?
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Answer
The investigation could be improved by doing the experiment at 30 °C, 35 °C, and 40 °C.
Step 5
Describe how the student could investigate the effect of pH on the breakdown of starch by amylase.
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To investigate the effect of pH on starch breakdown by amylase, the student could set up a series of test tubes with starch solution and buffered solutions at different pH levels (e.g., pH 4, 6, 7, 8). Then, they would add a fixed amount of amylase to each test tube and incubate them at a constant temperature. Samples could be taken at regular intervals to test for the presence of glucose, using iodine to check for starch breakdown. The amount of glucose produced at each pH level would indicate the effect of pH on amylase activity.