A student investigated the effect of enzyme concentration on the mass of product formed in one hour - Edexcel - GCSE Biology Combined Science - Question 3 - 2018 - Paper 1

The mass of product formed increases as enzyme concentration increases. This shows that enzyme activity is directly proportional to concentration up to a certain point. After reaching 30 arbitrary units, the mass of product formed levels off at 40 grams, indicating that the reaction has reached its maximum capacity for the enzyme activity.

Given the enzyme concentration of 40 arbitrary units corresponds to 40 grams of product, the ratio is:

$\text{Ratio} = \frac{\text{enzyme concentration}}{\text{mass of product}} = \frac{5}{15} = 1:3$

D increase the substrate concentration. Increasing the amount of substrate can lead to more product formation, as it allows more opportunities for the enzyme to catalyze the reaction.

At 80°C, the enzyme pepsin would denature, losing its functional shape. This denaturation prevents the enzyme-substrate complexes from forming effectively, leading to little or no product formation. Therefore, such a high temperature is not suitable for enzyme activity.