Figure 3 outlines a method that can be used to extract DNA from fruit - Edexcel - GCSE Biology Combined Science - Question 2 - 2016 - Paper 1
Question 2
Figure 3 outlines a method that can be used to extract DNA from fruit.
Crush fruit with a buffer solution containing detergent
Filter the mixture
Add ethanol and ... show full transcript
Worked Solution & Example Answer:Figure 3 outlines a method that can be used to extract DNA from fruit - Edexcel - GCSE Biology Combined Science - Question 2 - 2016 - Paper 1
Step 1
Give a reason for filtering the mixture.
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Answer
Filtering the mixture is necessary to separate solid material, such as fruit pulp and cell debris, from the liquid. This allows for a clearer solution that contains the dissolved DNA and prevents impurities from interfering with subsequent steps in the extraction process.
Step 2
What is the role of the ethanol?
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Answer
The role of the ethanol in the DNA extraction process is to precipitate the DNA. Ethanol, when added to the aqueous solution containing DNA, causes the DNA to become insoluble and aggregate for easier collection.
