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A student investigated the antibiotic properties of garlic - Edexcel - GCSE Biology - Question 5 - 2023 - Paper 1

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A student investigated the antibiotic properties of garlic. Bacteria were spread on an agar plate. A paper disc was soaked in garlic extract and placed on this agar ... show full transcript

Worked Solution & Example Answer:A student investigated the antibiotic properties of garlic - Edexcel - GCSE Biology - Question 5 - 2023 - Paper 1

Step 1

Suggest why there is an area with no bacterial growth.

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Answer

The area with no bacterial growth suggests that the garlic extract has antibiotic properties which effectively killed the bacteria in that region.

Step 2

Explain which agar plate, shown in Figure 10, has the paper disc containing the 100 mg/cm² garlic extract.

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Answer

Agar plate C is the one with the paper disc containing the 100 mg/cm² garlic extract, as it shows the largest clear zone with no microbial growth, indicating the highest antibacterial activity.

Step 3

Give one way the student could improve their results.

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Answer

One way the student could improve their results is by ensuring that the agar plates are incubated at the same temperature during the experiment.

Step 4

Calculate the area with no bacterial growth for agar plate B.

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Answer

To find the area, we use the formula for the area of a circle: ext{Area} = rac{22}{7} r^2 Substituting the radius (r = 5 mm): ext{Area} = rac{22}{7} imes (5^2) = rac{22}{7} imes 25 = 78.5 ext{ mm}^2

Step 5

State the reason for heating the spreader in the flame of a Bunsen burner before use.

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Answer

The spreader is heated to sterilise it, which helps to eliminate any bacteria or microorganisms present on the spreader.

Step 6

Give two other ways of working safely with microorganisms.

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Answer

  1. Disinfect the work surface before starting the experiment.
  2. Use protective gloves or a face mask when handling microorganisms.

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