During recombinant DNA technology, restriction endonucleases are used to cut DNA into fragments - Scottish Highers Biology - Question 16 - 2022
Question 16
During recombinant DNA technology, restriction endonucleases are used to cut DNA into fragments. Each endonuclease recognises a specific DNA sequence called a restri... show full transcript
Worked Solution & Example Answer:During recombinant DNA technology, restriction endonucleases are used to cut DNA into fragments - Scottish Highers Biology - Question 16 - 2022
Step 1
EcoRI
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Answer
EcoRI recognizes its specific DNA sequence and cuts at that site, producing 2 fragments. This can be determined by analyzing the diagram and counting the number of sections created by the cut.
Step 2
HhaI
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Answer
Similarly, HhaI cuts once in this section, corresponding to 3 fragments. Again, we can confirm this by evaluating the number of resulting sections after the cuts.
Step 3
EcoRI + HhaI
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Answer
When both EcoRI and HhaI are used together, the number of DNA fragments produced will be 6. This is a cumulative result of all cuts made by both endonucleases as indicated in the table.
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