Scottish Highers Biology Genetic Control of Metabolism: The diagram shows some features

Question

The diagram shows some features of a plasmid which has been cut open by a restriction endonuclease to allow a gene from a donor chromosome to be inserted.

The DNA recognition sites for three restriction endonucleases, EcoR1, BamH1, and HindIII, are shown in the table. The arrows indicate where each restriction endonuclease cuts the DNA sequence.

(i) A restriction endonuclease was used to remove a gene from a donor chromosome.
Use information from the diagram and the table to identify the restriction endonuclease which would be used to allow the gene to be inserted into the plasmid.
Give a reason for your answer.

Restriction endonuclease

Reason

(ii) Name the enzyme which would be used to seal the gene into the plasmid.

(iii) A culture of bacterial cells, 20% of which had taken up this modified plasmid, were grown on a nutrient agar plate. The plate was incubated and 250 colonies of this bacteria grew.
Predict the number of colonies which would have been expected to grow if the nutrient agar plate had contained the antibiotic ampicillin.

Space for calculation

(b) Name region X, shown in the diagram, which ensured that the modified plasmid would be passed on to daughter cells.

The diagram shows some features of a plasmid which has been cut open by a restriction endonuclease to allow a gene from a donor chromosome to be inserted - Scottish Highers Biology - Question 5 - 2018

Worked Solution & Example Answer:The diagram shows some features of a plasmid which has been cut open by a restriction endonuclease to allow a gene from a donor chromosome to be inserted - Scottish Highers Biology - Question 5 - 2018

Identify the restriction endonuclease to allow gene insertion

Name the enzyme which would be used to seal the gene into the plasmid

Predict the number of colonies which would have been expected to grow with ampicillin

Name region X to ensure plasmid inheritance

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