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Enzymes can be used to cut strands of DNA into fragments - Scottish Highers Human Biology - Question 6 - 2019

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Enzymes can be used to cut strands of DNA into fragments. The diagram shows the cutting sites of two different enzymes on a DNA strand that is 40 kilobases (kb) long... show full transcript

Worked Solution & Example Answer:Enzymes can be used to cut strands of DNA into fragments - Scottish Highers Human Biology - Question 6 - 2019

Step 1

What length of fragments (kb) would be produced if the DNA strand was cut with enzyme X only?

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Answer

To determine the lengths of the fragments produced by enzyme X, we first need to locate the cutting site of enzyme X on the 40 kb DNA strand. The diagram indicates that enzyme X cuts the strand into two segments.

  1. Identify Cutting Points: From the diagram, enzyme X cuts the DNA at the 12 kb mark.
  2. Calculate Fragment Lengths: The DNA strand is 40 kb long, and since enzyme X cuts at 12 kb:
    • The first fragment will be from 0 kb to 12 kb, which is 12 kb long.
    • The second fragment will be from 12 kb to 40 kb, which is calculated as: 40extkb12extkb=28extkb40 ext{ kb} - 12 ext{ kb} = 28 ext{ kb}

Thus, the lengths of the fragments produced by enzyme X are 12 kb and 28 kb.

Final Answer: B 12, 28

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