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Complete the following definitions - AQA - A-Level Biology - Question 4 - 2022 - Paper 3

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Complete the following definitions. The genome is The proteome is Recombinant DNA technology can involve the transfer of fragments of human DNA into bacteria. T... show full transcript

Worked Solution & Example Answer:Complete the following definitions - AQA - A-Level Biology - Question 4 - 2022 - Paper 3

Step 1

The genome is

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Answer

The genome is the complete set of genes in a cell or organism, including all the DNA that makes up the genes and genetic material.

Step 2

The proteome is

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Answer

The proteome is the range of proteins that a cell or organism can produce, reflecting the functional elements expressed by the genome.

Step 3

Give two reasons why bacteria are able to use human DNA to produce human proteins.

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Answer

  1. Bacteria possess the necessary transcription and translation machinery to process human DNA and produce proteins.

  2. The genetic code is universal, meaning that bacteria can interpret human genes to synthesize corresponding proteins.

Step 4

Suggest and explain one reason why bacteria might not be able to produce every human protein.

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Answer

Bacteria may not have the appropriate post-translational modification machinery required to modify some human proteins correctly, affecting their functionality. For example, some proteins require specific glycosylation that only occurs in eukaryotic cells.

Step 5

Name regions M and N shown in Figure 7.

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Answer

Region M is the promoter, which initiates transcription of the antithrombin gene.

Region N is the terminator, which signals the end of transcription.

Step 6

Explain the purpose of the marker gene.

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Answer

The marker gene allows for the identification of cells that have successfully incorporated the antithrombin gene, ensuring that genetic modifications can be tracked and confirmed.

Step 7

Suggest two explanations for the importance of the enhancer being included in the DNA fragment transferred.

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Answer

  1. The enhancer increases the transcriptional activity of the antithrombin gene in specific tissues, enhancing protein production.

  2. The enhancer ensures that the antithrombin gene is expressed at the right developmental stage or in response to the right signals, which is crucial for efficient protein production.

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