Plasmids of bacteria are used to transfer selected genes from one species to another. The process can be represented as follows. bacterial plasmid cut → foreign gene and plasmid mixed → plasmid with inserted foreign gene Enzymes are used to facilitate several of these steps. Which one of the following shows the enzymes required for the first and last steps of the process? Cuts plasmid Inserts genes A. restriction enzyme DNA ligase B. restriction enzyme DNA polymerase C. DNA ligase DNA polymerase D. DNA polymerase DNA ligase

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Plasmids of bacteria are used to transfer selected genes from one species to another - VCE - SSCE Biology - Question 25 - 2014 - Paper 1

Question 25

Plasmids of bacteria are used to transfer selected genes from one species to another. The process can be represented as follows. bacterial plasmid cut → foreign ge... show full transcript

Worked Solution & Example Answer:Plasmids of bacteria are used to transfer selected genes from one species to another - VCE - SSCE Biology - Question 25 - 2014 - Paper 1

Step 1

Cuts plasmid

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Answer

To cut the plasmid, a restriction enzyme is required. This enzyme recognizes specific sequences in the DNA and creates cuts at those sites, enabling the insertion of the foreign gene.

Step 2

Inserts genes

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Answer

To insert the foreign gene into the plasmid, DNA ligase is necessary. This enzyme joins the cut ends of the DNA, facilitating the stable incorporation of the foreign gene into the plasmid.

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Plasmids of bacteria are used to transfer selected genes from one species to another - VCE - SSCE Biology - Question 25 - 2014 - Paper 1

Worked Solution & Example Answer:Plasmids of bacteria are used to transfer selected genes from one species to another - VCE - SSCE Biology - Question 25 - 2014 - Paper 1

Cuts plasmid

Inserts genes

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