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Scientists have been able to use biotechnology to ‘cut and paste’ DNA for decades - HSC - SSCE Biology - Question 20 - 2019 - Paper 1
Question 20
Scientists have been able to use biotechnology to ‘cut and paste’ DNA for decades. Why would the new CRISPR/Cas9 technology have improved the scientists' success in... show full transcript
Worked Solution & Example Answer:Scientists have been able to use biotechnology to ‘cut and paste’ DNA for decades - HSC - SSCE Biology - Question 20 - 2019 - Paper 1
Step 1
D. gRNA has nucleotides complementary to the target DNA.
Answer
The CRISPR/Cas9 system significantly enhances gene editing precision. One key component, the guide RNA (gRNA), plays a crucial role. gRNA is designed to have nucleotides that are complementary to the target DNA sequence.
This complementarity allows gRNA to bind specifically to the target DNA. Once the gRNA is bound to its complementary DNA, the Cas9 enzyme can locate this bound complex and induce a double strand break at the specified location.
Therefore, the correct answer is that gRNA has nucleotides complementary to the target DNA, which enables precise cutting of specific genes.