The following are the five steps in the process of gene cloning - HSC - SSCE Biology - Question 19 - 2023 - Paper 1
Question 19
The following are the five steps in the process of gene cloning.
1. Selection of organisms containing recombinant DNA sequences
2. Creation of recombinant DNA joined... show full transcript
Worked Solution & Example Answer:The following are the five steps in the process of gene cloning - HSC - SSCE Biology - Question 19 - 2023 - Paper 1
Step 1
5. Choice of host organism and cloning vector
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Choosing the appropriate host organism and cloning vector is crucial as it determines how well the recombinant DNA will be inserted and expressed.
Step 2
4. Extraction and amplification of DNA to be cloned
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After selecting the host, extract the DNA that you want to clone and amplify it to ensure you have enough material for the next steps.
Step 3
2. Creation of recombinant DNA joined using DNA ligase
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Join the amplified DNA with the vector using DNA ligase, facilitating the formation of recombinant DNA.
Step 4
1. Selection of organisms containing recombinant DNA sequences
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Finally, select the organisms that carry the recombinant DNA to verify successful cloning and expression.
Step 5
3. Introduction of recombinant DNA into host organism
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Transfer the recombinant DNA into your chosen host organism to initiate the cloning process.
