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Scientists have been able to use biotechnology to ‘cut and paste’ DNA for decades - HSC - SSCE Biology - Question 20 - 2019 - Paper 1
Question 20
Scientists have been able to use biotechnology to ‘cut and paste’ DNA for decades. Why would the new CRISPR/Cas9 technology have improved the scientists' success in... show full transcript
Worked Solution & Example Answer:Scientists have been able to use biotechnology to ‘cut and paste’ DNA for decades - HSC - SSCE Biology - Question 20 - 2019 - Paper 1
Step 1
D. gRNA has nucleotides complementary to the target DNA.
Answer
The CRISPR/Cas9 system significantly enhances the ability of scientists to cut specific DNA sequences. The guide RNA (gRNA) plays a crucial role by ensuring specificity in targeting. When the gRNA is designed to have nucleotides complementary to a specific region of target DNA, it allows the Cas9 protein to accurately bind to that exact location. This complementary binding is essential as it provides the precision needed to ensure that only the desired gene is targeted and modified, thereby improving overall gene-editing efficacy.