Jelly Bush honey has been used by Aboriginal Peoples to treat cuts, sores and burns - HSC - SSCE Biology - Question 26 - 2022 - Paper 1
Question 26
Jelly Bush honey has been used by Aboriginal Peoples to treat cuts, sores and burns. Recent studies have shown that Jelly Bush honey has a very high level of methylg... show full transcript
Worked Solution & Example Answer:Jelly Bush honey has been used by Aboriginal Peoples to treat cuts, sores and burns - HSC - SSCE Biology - Question 26 - 2022 - Paper 1
Step 1
Select appropriate safety equipment including gloves and safety clothing.
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It is crucial to ensure that the scientist and anyone involved in the experiment are protected from potential hazards. Wearing gloves and safety clothing minimizes the risk of contamination and ensures safe handling of materials.
Step 2
Prepare three nutrient agar plates, using sterile techniques. Seal one plate and label as control.
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Utilizing three agar plates helps in comparing the effectiveness of Jelly Bush honey against a control sample without honey. Sterile procedures are necessary to avoid introducing external contaminants.
Step 3
Inoculate the remaining plates with bacteria to cover the agar surface.
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This involves evenly spreading a bacterial culture on the agar plates to create a uniform layer, which provides a consistent background for assessing the impact of Jelly Bush honey.
Step 4
Place a sterile paper disc soaked in Jelly Bush honey in the middle of an agar plate. Seal and label as 'Honey'.
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The sterile disc allows the concentration of Jelly Bush honey to diffuse into the agar, creating a gradient and enabling the evaluation of its antibacterial effects.
Step 5
Incubate the plates at 25°C for 48 hours.
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Incubating at the specified temperature allows for optimal bacterial growth and the assessment of the effectiveness of Jelly Bush honey in inhibiting this growth.
Step 6
Remove from incubator but do not move any of the plates after incubation.
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Keeping the plates undisturbed ensures accurate measurement of the zone of inhibition around the Jelly Bush honey disc.
Step 7
Observe the control plate to check for any contaminating bacteria.
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This step is essential to confirm any growth observed in the experimental plate is due to the infection and not a result of contamination.
Step 8
For each agar plate, measure the zone of inhibition from edge to edge. Repeat the experiment three more times and calculate an average.
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Measuring the zones of inhibition helps quantify the effectiveness of Jelly Bush honey. Repeating the experiment increases reliability and provides an average value for more robust results.
