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Yeast is a single-celled fungus that can reproduce by budding - HSC - SSCE Biology - Question 27 - 2019 - Paper 1
Question 27
Yeast is a single-celled fungus that can reproduce by budding. (a) What type of reproduction is budding? (b) Outline a procedure that could be used to test the eff... show full transcript
Worked Solution & Example Answer:Yeast is a single-celled fungus that can reproduce by budding - HSC - SSCE Biology - Question 27 - 2019 - Paper 1
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(b) Outline a procedure that could be used to test the effect of temperature on reproduction in yeast.
Answer
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Prepare a suspension of yeast cells.
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Prepare water baths at three temperatures: 15°C, 25°C, and 35°C.
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Mix the suspension thoroughly to ensure even distribution.
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Measure and place 5 mL of suspension into 9 test tubes (3 tubes for each temperature).
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Place three test tubes in each water bath.
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Cover the test tubes with a coverslip.
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Take a drop from each test tube and place it on a microscope slide with a coverslip to observe under a microscope.
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Record the number of cells in each drop under a microscope after a set time (e.g., 24 hours).
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Compare the number of cells recorded at each temperature.