After 1 hour, the student filtered the samples - AQA - A-Level Biology - Question 4 - 2020 - Paper 1
Question 4
After 1 hour, the student filtered the samples.
She placed the filtrate in a colorimeter and measured the light absorbance.
Her results are shown in Figure 4.
Figur... show full transcript
Worked Solution & Example Answer:After 1 hour, the student filtered the samples - AQA - A-Level Biology - Question 4 - 2020 - Paper 1
Step 1
Higher absorbance indicates more anthocyanin
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Answer
The results in Figure 4 show varying levels of absorbance. A higher absorbance reading indicates that a greater concentration of anthocyanin is present in the solution, as more light is absorbed by the pigment.
Step 2
Higher absorbance indicates more membrane damage/permeability
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Answer
Additionally, a higher absorbance can suggest that the extraction process has resulted in greater membrane damage or permeability. This damage to the cell membranes allows more anthocyanin to be released into the solvent used.
Step 3
Extraction with different solvents
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In the case of the results shown, the readings for extraction solvents E and F differ; the higher absorbance for solvent E indicates it may better disrupt cell membranes, releasing more anthocyanin compared to solvent G.
Step 4
Comparison of solvents
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This data suggests that the efficiency of different solvents in extracting anthocyanin varies, impacting both the concentration of anthocyanin extracted and the extent of membrane disruption during the extraction process.
