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The student added 100 mm³ of each bacterial culture from its glass bottle onto a separate agar plate - AQA - A-Level Biology - Question 10 - 2023 - Paper 1
Question 10
The student added 100 mm³ of each bacterial culture from its glass bottle onto a separate agar plate. She spread each bacterial culture evenly over the agar using a ... show full transcript
Worked Solution & Example Answer:The student added 100 mm³ of each bacterial culture from its glass bottle onto a separate agar plate - AQA - A-Level Biology - Question 10 - 2023 - Paper 1
Step 1
Describe the aseptic techniques she should use.
Answer
To ensure the aseptic technique is followed, the student should:
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Sterilize Equipment: Use heat to sterilize the spreader before and after contact with the agar plates by passing it through a flame.
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Disinfect Surfaces: Clean the work surface with disinfectant to minimize contamination.
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Use a Bunsen Burner: Work near a Bunsen burner to create an updraft that reduces the risk of airborne contaminants settling on the agar plates.