A student used chromatography to separate the different photosynthetic pigments in a chlorophyll solution - AQA - A-Level Biology - Question 1 - 2023 - Paper 2
Question 1
A student used chromatography to separate the different photosynthetic pigments in a chlorophyll solution. She had the following materials.
Chromatography paper
A r... show full transcript
Worked Solution & Example Answer:A student used chromatography to separate the different photosynthetic pigments in a chlorophyll solution - AQA - A-Level Biology - Question 1 - 2023 - Paper 2
Step 1
Draw the origin line
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Answer
Using a ruler and pencil, draw a horizontal line near the bottom of the chromatography paper. This will serve as the origin line where the chlorophyll solution will be applied.
Step 2
Apply the chlorophyll solution
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Using a pipette or dropper, carefully add 2 cm³ of the chlorophyll solution onto the origin line drawn on the chromatography paper.
Step 3
Add solvent A
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Place the chromatography paper into suitable glassware containing solvent A. Ensure that the origin line is above the level of the solvent to prevent the chlorophyll solution from dissolving directly into the solvent.
Step 4
Stop the process
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Once the solvent front has moved up the paper, remove the chromatography paper from the glassware before the solvent reaches the top. Mark the position where the solvent front reached.
