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The student added 100 mm³ of each bacterial culture from its glass bottle onto a separate agar plate - AQA - A-Level Biology - Question 10 - 2023 - Paper 1
Question 10
The student added 100 mm³ of each bacterial culture from its glass bottle onto a separate agar plate. She spread each bacterial culture evenly over the agar using a ... show full transcript
Worked Solution & Example Answer:The student added 100 mm³ of each bacterial culture from its glass bottle onto a separate agar plate - AQA - A-Level Biology - Question 10 - 2023 - Paper 1
Step 1
Describe the aseptic techniques she should use.
Answer
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Sterilize Equipment: Before starting, all equipment such as the spreader and agar plates must be sterilized using an autoclave or by flaming to eliminate any unwanted microorganisms.
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Use Personal Protective Equipment (PPE): The student should wear gloves and a lab coat to prevent contamination of the cultures and protect herself.
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Work Near a Bunsen Burner: Conduct the procedure near a lit Bunsen burner to create an updraft that reduces the risk of airborne contaminants affecting the cultures.