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Calculate the mean stomatal density in the three fields of view in Figure 9 - AQA - A-Level Biology - Question 9 - 2020 - Paper 1

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Question 9

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Calculate the mean stomatal density in the three fields of view in Figure 9. Give your answer as number of stomata per mm². Show your working.

Worked Solution & Example Answer:Calculate the mean stomatal density in the three fields of view in Figure 9 - AQA - A-Level Biology - Question 9 - 2020 - Paper 1

Step 1

Calculate the area of one square from the field of view

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Answer

Each edge of the white square in Figure 9 measures 250 µm. To find the area in mm², convert 250 µm to mm: 250 µm = 0.25 mm. Hence, the area of one square is:

Area=(0.25extmm)2=0.0625extmm2Area = (0.25 ext{ mm})^2 = 0.0625 ext{ mm}^2

Step 2

Count the number of stomata in each field of view

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Answer

Assuming the following counts are obtained from the three fields of view:

  • Field 1: 10 stomata
  • Field 2: 11 stomata
  • Field 3: 8 stomata

Total stomata count = 10 + 11 + 8 = 29 stomata.

Step 3

Calculate the mean stomatal density

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Answer

Mean stomatal density can be calculated using the formula:

Meanextstomataldensity=TotalextstomataTotalextareainmm2Mean ext{ stomatal density} = \frac{Total ext{ stomata}}{Total ext{ area in } mm^2}

In our case, the total area for three squares is:

Totalextarea=3imes0.0625extmm2=0.1875extmm2Total ext{ area} = 3 imes 0.0625 ext{ mm}^2 = 0.1875 ext{ mm}^2

Now substituting the values:

Meanextstomataldensity=290.1875154.67extstomatapermm2Mean ext{ stomatal density} = \frac{29}{0.1875} \approx 154.67 ext{ stomata per mm}^2

After rounding, the final answer is approximately 155 stomata per mm².

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