A student used chromatography to separate the different photosynthetic pigments in a chlorophyll solution - AQA - A-Level Biology - Question 1 - 2023 - Paper 2
Question 1
A student used chromatography to separate the different photosynthetic pigments in a chlorophyll solution. She had the following materials.
Chromatography paper
A r... show full transcript
Worked Solution & Example Answer:A student used chromatography to separate the different photosynthetic pigments in a chlorophyll solution - AQA - A-Level Biology - Question 1 - 2023 - Paper 2
Step 1
Draw line/origin on chromatography paper
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Answer
Using a ruler and pencil, draw a horizontal line towards the bottom of the chromatography paper. This will serve as the origin for applying the chlorophyll solution.
Step 2
Add chlorophyll solution to origin
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Using a pipette or dropper, carefully place 2 cm³ of the chlorophyll solution on the drawn line without letting it spread too much.
Step 3
Add solvent A
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Next, lower the chromatography paper into a suitable container that holds solvent A, ensuring that the solvent does not touch the chlorophyll spot.
Step 4
Remove/stop the process
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Once the solvent front reaches an appropriate distance from the origin line, remove the paper from the solvent. Mark the position where the solvent front reached to analyze the results.
