To show the presence of micro-organisms in the roots of a clover plant. - Leaving Cert Agricultural Science - Question c - 2019
Question c
To show the presence of micro-organisms in the roots of a clover plant.
Worked Solution & Example Answer:To show the presence of micro-organisms in the roots of a clover plant. - Leaving Cert Agricultural Science - Question c - 2019
Step 1
Swab bench with disinfectant or work near lit Bunsen.
96%
114 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
Ensure a clean working area to prevent contamination.
Step 2
Prepare sterile agar plates.
99%
104 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
Use two sterile agar plates for culturing the microorganisms.
Step 3
Wash soil off roots.
96%
101 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
Carefully remove the clover plant and wash the roots to eliminate any soil contaminants.
Step 4
Dip in mild disinfectant solution.
98%
120 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
Soak the washed roots in a mild disinfectant solution to reduce microbial load without killing the organisms of interest.
Step 5
Crush nodules.
97%
117 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
Crush the nodules found on the roots to release potential microorganisms into the culture medium.
Step 6
Streak on agar plate with inoculating loop.
97%
121 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
Use an inoculating loop to transfer the crushed material onto the agar plate, streaking it for even distribution.
Step 7
Incubate for at least 5 days at 20–30 °C.
96%
114 rated
Only available for registered users.
Sign up now to view full answer, or log in if you already have an account!
Answer
Place the agar plates in an incubator at a temperature between 20 and 30 degrees Celsius for a minimum of 5 days to allow colonies to grow.
Join the Leaving Cert students using SimpleStudy...
