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Silage making involves the activity of anaerobic bacteria - Leaving Cert Agricultural Science - Question a - 2014

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Silage making involves the activity of anaerobic bacteria. (i) Explain the term anaerobic. (ii) Name two species of bacteria that may be involved in silage making.... show full transcript

Worked Solution & Example Answer:Silage making involves the activity of anaerobic bacteria - Leaving Cert Agricultural Science - Question a - 2014

Step 1

Explain the term anaerobic.

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Answer

The term 'anaerobic' refers to an environment that lacks oxygen. In anaerobic processes, organisms or bacteria thrive in the absence of air, relying instead on alternative metabolic pathways for energy production.

Step 2

Name two species of bacteria that may be involved in silage making.

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Answer

Two species of bacteria that are commonly involved in silage making are Lactobacillus and Clostridium.

Step 3

Describe two ways of ensuring anaerobic conditions in the silage pit.

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Answer

  1. Rolling or Quick Filling: Quickly filling the silage pit helps to minimize the exposure of the material to air by compressing the contents.
  2. Covering and Avoiding Air Pockets: After filling, covering the silage with a tarp or using tyres on top helps to seal the air out, preventing aerobic bacteria from entering.

Step 4

Describe an experiment to show the presence of bacteria in a sample of silage.

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Answer

To demonstrate the presence of bacteria in silage, take two sterile agar plates and use a flaming loop to transfer a sample of the silage onto one plate. Leave the other plate as a control. Seal both plates and incubate them in an oven set to 20–30°C for 2–3 days. After incubation, examine the plates; the bacteria from the silage will appear as shiny spots, while the control plate will show an absence of spots.

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