In relation to an investigation you carried out into heat denaturation of an enzyme, answer the following:
1 - Leaving Cert Biology - Question c - 2015
Question c
In relation to an investigation you carried out into heat denaturation of an enzyme, answer the following:
1. Name the enzyme you used. ____________________________... show full transcript
Worked Solution & Example Answer:In relation to an investigation you carried out into heat denaturation of an enzyme, answer the following:
1 - Leaving Cert Biology - Question c - 2015
Step 1
1. Name the enzyme you used.
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Answer
Catalase
Step 2
2. Name the enzyme's substrate.
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Answer
Hydrogen peroxide
Step 3
3. Name the product(s) formed.
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Answer
Oxygen (and water)
Step 4
ii) How did you denature the enzyme?
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Answer
I boiled the enzyme or heated it to a high temperature (≥ 60 °C).
Step 5
iii) How did you know that the enzyme had been denatured?
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Answer
There was a negative result for the named test for oxygen, indicating that the enzyme had been denatured.
Step 6
iv) Why are buffers needed when carrying out experiments with enzymes in school?
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Answer
Buffers are needed to maintain a constant pH or to vary pH.
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