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(a) (i) What term is used for the substance(s) that result(s) from the action of an enzyme on its substrate? (ii) In relation to an enzyme, explain the term optimum activity - Leaving Cert Biology - Question 8 - 2013
Question 8
(a) (i) What term is used for the substance(s) that result(s) from the action of an enzyme on its substrate? (ii) In relation to an enzyme, explain the term optim... show full transcript
Worked Solution & Example Answer:(a) (i) What term is used for the substance(s) that result(s) from the action of an enzyme on its substrate? (ii) In relation to an enzyme, explain the term optimum activity - Leaving Cert Biology - Question 8 - 2013
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In relation to an enzyme, explain the term optimum activity.
Answer
In the context of an enzyme, 'optimum activity' refers to the specific conditions (such as temperature and pH) under which the enzyme catalyzes reactions at its maximum rate. Enzymes have a preferred range of conditions that allow them to function most efficiently, beyond which their activity may decline due to denaturation or changes in the active site.
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Describe how you carried out the investigation. In your description outline how you measured the activity of the enzyme.
Answer
To investigate the effect of heat denaturation on enzyme activity, I placed a test solution containing amylase and starch in a water bath. I heated the solution to temperatures above 60°C for at least 5 minutes. After cooling, I conducted a control experiment using untreated amylase. The activity was measured by testing for the presence of reducing sugars using Benedict's reagent, comparing the results of treated versus untreated enzymes to assess the effects of heat.
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