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9. (a) (i) Give any one base pair found in DNA - Leaving Cert Biology - Question 9 - 2015

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9. (a) (i) Give any one base pair found in DNA. (ii) Mutations are changes in DNA or chromosomes. State one cause of mutation. (b) Answer the following in rel... show full transcript

Worked Solution & Example Answer:9. (a) (i) Give any one base pair found in DNA - Leaving Cert Biology - Question 9 - 2015

Step 1

Give any one base pair found in DNA.

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Answer

One base pair found in DNA is Adenine (A) and Thymine (T).

Step 2

State one cause of mutation.

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Answer

One cause of mutation can be radiation or chemicals such as cigarette smoke.

Step 3

Name a suitable plant for this activity.

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Answer

A suitable plant for this activity is Onion.

Step 4

Indicate below, using the letters A, B, C and D, the correct order of the above steps.

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Answer

Step 1: C (Chop the plant tissue into small pieces.) Step 2: B (Add the mixture of the plant tissue, salt and washing up liquid to a blender.) Step 3: D (Add 2 – 3 drops of protease to the filtered plant extract.) Step 4: A (Pour freezer-cold ethanol down the inside of the test tube containing the filtered plant extract.)

Step 5

Why was the protease added to the filtered plant extract?

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Answer

The protease was added to break down proteins in the chromosomes, enabling easier access to the DNA.

Step 6

What was the purpose of adding washing up liquid to the mixture?

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Answer

The washing up liquid was added to break down cell membranes, allowing the release of DNA.

Step 7

What was the purpose of the freezer-cold ethanol?

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Answer

The purpose of the freezer-cold ethanol was to bring DNA out of solution or to allow DNA to be seen.

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