8. (a) (i) What is meant by the term germination?
(ii) State three factors necessary for successful germination - Leaving Cert Biology - Question 8 - 2020
Question 8
8.
(a) (i) What is meant by the term germination?
(ii) State three factors necessary for successful germination.
(b) Answer the following questions in relation to... show full transcript
Worked Solution & Example Answer:8. (a) (i) What is meant by the term germination?
(ii) State three factors necessary for successful germination - Leaving Cert Biology - Question 8 - 2020
Step 1
What is meant by the term germination?
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Answer
Germination is the process of growth or development of a seed into a new plant. It involves the emergence of the embryo from the seed and its subsequent growth into a seedling.
Step 2
State three factors necessary for successful germination.
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Answer
The three factors necessary for successful germination are:
Oxygen
Water
Suitable temperature
Step 3
State whether you used starch agar or skimmed milk (protein) agar.
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Answer
I used skimmed milk (protein) agar for the investigation.
Step 4
Why were the seeds soaked in water prior to the investigation?
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The seeds were soaked in water to start germination by breaking dormancy, activating enzymes, and allowing for nutrient absorption to initiate metabolism.
Step 5
Describe how you further prepared the seeds and added them to the agar plate(s).
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I split the seeds into two halves to facilitate the exposure of the inner tissues. Next, I sterilized the seeds by soaking them in disinfectant for a short period. Then, I placed them on the agar, ensuring they lay flat and did not overlap.
Step 6
Under what conditions did you store the plate(s) containing the seeds to allow germination to occur?
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The plates were stored in an incubator at a suitable temperature of around 20–25°C for a period of 1 to 7 days.
Step 7
How did you test to show that digestion had occurred?
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I added iodine to the agar plate to test for any digestive activity. If starch was digested, the iodine would not react with the area where digestion occurred.
Step 8
State the result that showed digestion had occurred.
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The result that indicated digestion had occurred was a clear zone in the agar surrounding the seeds. The starch digested by enzymes would show a change, where the iodine did not change the color of that area.
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