In relation to the isolation of DNA from a plant tissue, explain why you used each of the following:
1 - Leaving Cert Biology - Question b (i) - 2015
Question b (i)
In relation to the isolation of DNA from a plant tissue, explain why you used each of the following:
1. Washing-up or similar liquid.
2. Sodium chloride.
3. Prote... show full transcript
Worked Solution & Example Answer:In relation to the isolation of DNA from a plant tissue, explain why you used each of the following:
1 - Leaving Cert Biology - Question b (i) - 2015
Step 1
1. Washing-up or similar liquid.
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Answer
The washing-up liquid is used to break down the cell membranes. This helps to release the DNA contained within the cells.
Step 2
2. Sodium chloride.
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Answer
Sodium chloride causes the DNA to clump together, facilitating its extraction and making it easier to work with.
Step 3
3. Protease.
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Answer
Protease is used to break down or digest the proteins that are associated with the DNA in the chromosomes, ensuring a purer sample of DNA.
Step 4
4. Freezer-cold ethanol.
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Answer
Freezer-cold ethanol helps to bring the DNA out of solution, making it visible or aiding in the separation of the DNA from the rest of the cellular components.
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