In relation to the isolation of DNA from a plant tissue, explain why you used each of the following:
1 - Leaving Cert Biology - Question 7(b)(i) - 2015
Question 7(b)(i)
In relation to the isolation of DNA from a plant tissue, explain why you used each of the following:
1. Washing-up or similar liquid.
2. Sodium chloride.
3. Prote... show full transcript
Worked Solution & Example Answer:In relation to the isolation of DNA from a plant tissue, explain why you used each of the following:
1 - Leaving Cert Biology - Question 7(b)(i) - 2015
Step 1
1. Washing-up or similar liquid.
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Answer
Washing-up liquid is used to break down the cell membranes. This action disrupts the lipid bilayer and facilitates the release of cellular components, including DNA, into the solution.
Step 2
2. Sodium chloride.
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Answer
Sodium chloride is used to cause the DNA to clump together. The salt helps to neutralize the negative charges on the DNA molecules, allowing them to aggregate and become more visible.
Step 3
3. Protease.
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Answer
Protease is essential for breaking down or digesting proteins within the chromosomes. This process helps to remove histones and other proteins that may be associated with the DNA, thereby purifying the DNA sample.
Step 4
4. Freezer-cold ethanol.
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Answer
Freezer-cold ethanol is used to separate the DNA from the solution. When added to the mixture, the cold ethanol precipitates the DNA, making it visible and allowing for its collection.
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