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Describe how you carried out the investigation into the effect of IAA on plant tissue - Leaving Cert Biology - Question c - 2015
Question c
Describe how you carried out the investigation into the effect of IAA on plant tissue. What were the results of your investigation?
Worked Solution & Example Answer:Describe how you carried out the investigation into the effect of IAA on plant tissue - Leaving Cert Biology - Question c - 2015
Step 1
Describe how you carried out the investigation into the effect of IAA on plant tissue.
Answer
To investigate the effect of Indole-3-acetic acid (IAA) on plant tissue, I followed these steps:
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Experimental Setup: I selected plant tissues (e.g., cuttings from a specific plant species) and divided them into two groups: one exposed to IAA and the other not exposed to it. Alternatively, I could have used different concentrations of IAA.
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Application: I applied the IAA solution to the selected tissue, ensuring one group received no treatment (control group) for a clear comparison. I ensured that the tissues were properly immersed or coated with the solution.
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Incubation: I left the tissues in a suitable environment for at least 48 hours to allow the IAA to influence growth rates.
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Measurement: After the incubation, I measured the growth of the tissues using a ruler or suitable measuring device, comparing the growth in both groups. I recorded the results meticulously for analysis.
Step 2
What were the results of your investigation?
Answer
The results of my investigation indicated that the presence of IAA significantly influenced growth patterns in the plant tissues. The samples exposed to IAA demonstrated enhanced growth compared to the control group. Specifically, there was a noticeable increase in length and overall biomass of the tissues treated with IAA. Additionally, I observed variation in growth depending on the concentration of IAA used, indicating a dose-response relationship. Higher concentrations promoted more growth up to a certain level, after which growth may have plateaued or decreased, suggesting possible saturation or toxicity.