Mass spectrometry (MS), gas chromatography (GC), high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) are all used in analytical chemistry - Leaving Cert Chemistry - Question b - 2008
Question b
Mass spectrometry (MS), gas chromatography (GC), high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) are all used in analytical chemist... show full transcript
Worked Solution & Example Answer:Mass spectrometry (MS), gas chromatography (GC), high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) are all used in analytical chemistry - Leaving Cert Chemistry - Question b - 2008
Step 1
Mass spectrometry (MS)
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Answer
One important application of mass spectrometry is to identify the presence of isotopes, which can provide valuable information about the composition of various substances.
Step 2
Gas chromatography (GC)
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Answer
Gas chromatography is commonly used for testing the concentration of alcohol in blood samples.
Step 3
High-performance liquid chromatography (HPLC)
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Answer
HPLC is employed in separation techniques, particularly for analyzing vitamins and other important biomolecules.
Step 4
Thin-layer chromatography (TLC)
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TLC is utilized for the separation of dyes (pigments) in forensic science, helping to identify material components.
Step 5
Choose one of these analytical techniques and explain the principle upon which it is based.
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I choose Mass Spectrometry (MS). The principle of mass spectrometry is based on the movement of charged particles in a magnetic field. These charged particles are deflected according to their mass-to-charge ratio, allowing for the determination of their respective masses and the identification of the chemical structure of compounds.
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