Required Practical: Microscopy (OCR GCSE Biology A, Combined (Gateway Science Suite)): Flashcards

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Required Practical: Microscopy
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Stain used for plant cells (e.g., onion)

Iodine solution

Stain used for animal cells (e.g., cheek)

Methylene blue

Starting magnification when using microscope

Lowest magnification (e.g., 4x objective)

Knob for general focus on microscope

Coarse focus knob

Knob to sharpen microscope image

Fine focus knob

Formula for magnification

Magnification=Size of ImageReal Size of Object\text{Magnification} = \frac{\text{Size of Image}}{\text{Real Size of Object}}

Plant cell structures to identify

Cell wall, membrane, cytoplasm, nucleus, chloroplasts

Animal cell structures to identify

Cell membrane, cytoplasm, nucleus

Shape of plant cells

Regular shape

Shape of animal cells

Irregular shape

Unique features of plant cells

Cell wall, chloroplasts, large central vacuole

Animal cells: cell wall & chloroplasts present?

No cell wall or chloroplasts

Purpose of stains in microscopy

Enhance visibility of cell structures

What to avoid when placing coverslip

Air bubbles

Calculating total magnification

Objective lens ×\times eyepiece lens magnification

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