Required Practical: Food Tests (OCR GCSE Biology A (Gateway Science Suite)): Flashcards

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Required Practical: Food Tests
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Nutrient detected by Benedict's test

Reducing sugars

Benedict's test positive colour change

Blue to brick-red or orange

Benedict's test heating conditions

75°C for 5 minutes in hot water bath

Nutrient detected by iodine test

Starch

Iodine test positive colour change

Brown to blue-black

Nutrient detected by Biuret test

Proteins

Biuret test positive colour change

Blue to purple

Nutrient detected by emulsion test

Lipids

First step in emulsion test for lipids

Add 2 cm³ ethanol and shake

Second step in emulsion test for lipids

Add 2 cm³ distilled water

Emulsion test positive result

Cloudy white emulsion forms

Nutrient detected by Sudan III test

Lipids

Sudan III test positive result

Red-stained oil layer forms on surface

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