Action and Specificity (Leaving Cert Biology): Flashcards

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Action and Specificity
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Active site

Region where enzyme binds substrate and reactions occur

Active site shape

Complementary 3D shape to substrate

What % of enzyme is the active site?

10-20%

Induced fit model

Active site changes shape slightly when substrate binds

Enzyme-substrate complex

Enzyme and substrate bound together

Enzyme processing speed

Thousands of substrate molecules per second

Enzyme specificity

Each enzyme works with one substrate type only

Denaturation

Permanent loss of enzyme function from shape change

Temperature causing enzyme denaturation

Above 40°C

Catalase function

Breaks down hydrogen peroxide into water and oxygen

Sources of catalase

Radishes, celery, potatoes, most living organisms

Visible effect of catalase on hydrogen peroxide

Rapid foaming and bubbling as oxygen is released

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